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Platelets are fundamental effector cells of the haemostatic system, and their function can be modulated by peripheral tissues. In this study, we determined whether liver innervation modulated platelet-endothelial interactions. Hepatic nerve (HN) density (Ndh) was determined by immunohistochemistry in normal human liver tissue, and the response of human platelets to ex vivo acetylcholine (Ach) stimulation was determined by measuring platelet aggregation. The functional significance of liver-derived neurohumour factors was examined by examining Ach-induced platelet-endothelial interactions in mouse mesenteric venules, in vitro and in vivo. Ndh in normal human liver tissue ranged from 6.6 to 11.8 HN/mm(2). Ach-induced platelet aggregation was significantly (P be359ba680
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